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Cell Signaling Technology Inc nrf1
Nrf1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+nrf1/NRF1+Rabbit+mAb/pmc13036566-141-36-38
Average 95 stars, based on 119 article reviews

nrf1 - by Bioz Stars, 2026-09

95/100 stars

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Article Title: Orientia tsutsugamushi Ank5 directs ubiquitination and proteasomal degradation of NLRC5 to inhibit major histocompatibility complex class I expression
Article Snippet: .. Briefly, normalized amounts of eluates were resolved by SDS-PAGE in 4 to 15% TGX polyacrylamide gels (Bio-Rad) at 110 V for 15 min followed by 200 V for 25 min. Proteins were transferred onto nitrocellulose membrane in Towbin buffer at 100 V for 30 min. Blots were blocked in either 5% (vol/vol) non-fat dry milk or 5% (vol/vol) BSA in tris-buffered saline plus 0.05% Tween 20 (TBS-T) after which they were screened with rat anti-NLRC5 (MilliporeSigma [MABF260], 1:1,000), rabbit anti-TSA56 (1:1,000), rabbit or mouse anti-Flag (Sigma-Aldrich [F7425 or F1804], 1:1,000), rabbit anti-GFP (ThermoFisher Scientific, Rockford, IL [A-6455], 1:1,000), mouse anti-GAPDH (Santa Cruz, Dallas, TX [sc-365062], 1:750), rabbit or mouse anti-myc (Abcam, Cambridge, United Kingdom [ab9106 and ab32], 1:1,000), rabbit anti-HA (Abcam [ab9110], 1:4,000), mouse anti-HLA-ABC heavy chain (Abcam [ab70328], 1:1,000), rabbit anti-Β2M (Life Technologies, Carlsbad, CA [4H5L5], 1:1,000), rabbit anti-Skp1 (Cell Signaling Technology, Danvers, MA [2156S], 1:750), or rabbit anti-Nrf1 (Cell Signaling Technology [D5B10], 1:1,000). .. Bound primary antibodies were detected using horseradish peroxidase (HRP)-conjugated horse anti-mouse, anti-rabbit, and anti-rat IgG (Cell Signaling Technology, [7076S, 7074S, 7077S], 1:10,000).

Article Title: Orientia tsutsugamushi Ank5 promotes NLRC5 cytoplasmic retention and degradation to inhibit MHC class I expression
Article Snippet: .. Briefly, normalized amounts of eluates were resolved by SDS-PAGE in 4 to 15% TGX polyacrylamide gels (Bio-Rad) at 110 V for 15 min followed by 200 V for 25 min. Proteins were transferred onto nitrocellulose membrane in Towbin buffer at 100 V for 30 min. Blots were blocked in either 5% (vol/vol) non-fat dry milk or 5% (vol/vol) BSA in tris-buffered saline plus 0.05% Tween 20 (TBS-T) after which they were screened with rat anti-NLRC5 (MilliporeSigma [MABF260], 1:1000), rabbit anti-TSA56 (1:1000), rabbit or mouse anti-Flag (Sigma-Aldrich [F7425 or F1804], 1:1000), rabbit anti-GFP (ThermoFisher Scientific, Rockford, IL [A-6455], 1:1000), mouse anti-GAPDH (Santa Cruz, Dallas, TX [sc-365062], 1:750), rabbit or mouse anti-myc (Abcam, Cambridge, United Kingdom [ab9106 and ab32], 1:1000), rabbit anti-HA (Abcam [ab9110], 1:4000), mouse anti-HLA-ABC heavy chain (Abcam [ab70328], 1:1000), rabbit anti-β2 M (Life Technologies, Carlsbad, CA [4H5L5], 1:1000), rabbit anti-Skp1 (Cell Signaling Technology, Danvers, MA [2156S], 1:750), rabbit anti-Nrf1 (Cell Signaling Technology [D5B10], 1:1000), rabbit anti-lamin A/C (Cell Signaling [2032S]; 1:1000), or mouse anti-Hsp90 (Santa Cruz [13119]; 1:250). .. Bound primary antibodies were detected using horseradish peroxidase (HRP)-conjugated horse anti-mouse IgG, anti-rabbit IgG, and anti-rat IgG (Cell Signaling Technology, [7076S, 7074S, 7077S], 1:10,000).

Article Title: Orientia tsutsugamushi Ank5 directs ubiquitination and proteasomal degradation of NLRC5 to inhibit major histocompatibility complex class I expression
Article Snippet: .. Western blotting was performed as previously described.68 Briefly, normalized 593 amounts of eluates were resolved by SDS-PAGE in 4 to 15% TGX polyacrylamide gels (Bio-Rad) 594 at 110 V for 15 min followed by 200 V for 25 min. Proteins were transferred onto nitrocellulose 595 membrane in Towbin buffer at 100 V for 30 min. Blots were blocked in either 5% (vol/vol) non-596 fat dry milk or 5% (vol/vol) BSA in tris-buffered saline plus 0.05% Tween 20 (TBS-T) after which 597 they were screened with rat anti-NLRC5 (MilliporeSigma [MABF260], 1:1,000), rabbit anti-598 TSA5665 (1:1,000), rabbit or mouse anti-Flag (Sigma-Aldrich [F7425 or F1804], 1:1,000), rabbit 599 anti-GFP (ThermoFisher Scientific, Rockford, IL [A-6455], 1:1,000), mouse anti-GAPDH (Santa 600 Cruz, Dallas, TX [sc-365062], 1:750), rabbit or mouse anti-myc (Abcam, Cambridge, United 601 Kingdom [ab9106 and ab32], 1:1,000), rabbit anti-HA (Abcam [ab9110], 1:4,000), mouse anti-602 HLA-ABC heavy chain (Abcam [ab70328], 1:1,000), rabbit anti-b2M (Life Technologies, 603 Carlsbad, CA [4H5L5], 1:1,000), rabbit anti-Skp1 (Cell Signaling Technology, Danvers, MA 604 [2156S], 1:750), or rabbit anti-Nrf1 (Cell Signaling Technology [D5B10], 1:1,000). .. Bound 605 primary antibodies were detected using horseradish peroxidase (HRP)-conjugated horse anti-606 mouse, anti-rabbit, and anti-rat IgG (Cell Signaling Technology, [7076S, 7074S, 7077S], 607 1:10,000).

Membrane:

Article Title: Orientia tsutsugamushi Ank5 directs ubiquitination and proteasomal degradation of NLRC5 to inhibit major histocompatibility complex class I expression
Article Snippet: .. Western blotting was performed as previously described.68 Briefly, normalized 593 amounts of eluates were resolved by SDS-PAGE in 4 to 15% TGX polyacrylamide gels (Bio-Rad) 594 at 110 V for 15 min followed by 200 V for 25 min. Proteins were transferred onto nitrocellulose 595 membrane in Towbin buffer at 100 V for 30 min. Blots were blocked in either 5% (vol/vol) non-596 fat dry milk or 5% (vol/vol) BSA in tris-buffered saline plus 0.05% Tween 20 (TBS-T) after which 597 they were screened with rat anti-NLRC5 (MilliporeSigma [MABF260], 1:1,000), rabbit anti-598 TSA5665 (1:1,000), rabbit or mouse anti-Flag (Sigma-Aldrich [F7425 or F1804], 1:1,000), rabbit 599 anti-GFP (ThermoFisher Scientific, Rockford, IL [A-6455], 1:1,000), mouse anti-GAPDH (Santa 600 Cruz, Dallas, TX [sc-365062], 1:750), rabbit or mouse anti-myc (Abcam, Cambridge, United 601 Kingdom [ab9106 and ab32], 1:1,000), rabbit anti-HA (Abcam [ab9110], 1:4,000), mouse anti-602 HLA-ABC heavy chain (Abcam [ab70328], 1:1,000), rabbit anti-b2M (Life Technologies, 603 Carlsbad, CA [4H5L5], 1:1,000), rabbit anti-Skp1 (Cell Signaling Technology, Danvers, MA 604 [2156S], 1:750), or rabbit anti-Nrf1 (Cell Signaling Technology [D5B10], 1:1,000). .. Bound 605 primary antibodies were detected using horseradish peroxidase (HRP)-conjugated horse anti-606 mouse, anti-rabbit, and anti-rat IgG (Cell Signaling Technology, [7076S, 7074S, 7077S], 607 1:10,000).

Saline:

Article Title: Orientia tsutsugamushi Ank5 directs ubiquitination and proteasomal degradation of NLRC5 to inhibit major histocompatibility complex class I expression
Article Snippet: .. Western blotting was performed as previously described.68 Briefly, normalized 593 amounts of eluates were resolved by SDS-PAGE in 4 to 15% TGX polyacrylamide gels (Bio-Rad) 594 at 110 V for 15 min followed by 200 V for 25 min. Proteins were transferred onto nitrocellulose 595 membrane in Towbin buffer at 100 V for 30 min. Blots were blocked in either 5% (vol/vol) non-596 fat dry milk or 5% (vol/vol) BSA in tris-buffered saline plus 0.05% Tween 20 (TBS-T) after which 597 they were screened with rat anti-NLRC5 (MilliporeSigma [MABF260], 1:1,000), rabbit anti-598 TSA5665 (1:1,000), rabbit or mouse anti-Flag (Sigma-Aldrich [F7425 or F1804], 1:1,000), rabbit 599 anti-GFP (ThermoFisher Scientific, Rockford, IL [A-6455], 1:1,000), mouse anti-GAPDH (Santa 600 Cruz, Dallas, TX [sc-365062], 1:750), rabbit or mouse anti-myc (Abcam, Cambridge, United 601 Kingdom [ab9106 and ab32], 1:1,000), rabbit anti-HA (Abcam [ab9110], 1:4,000), mouse anti-602 HLA-ABC heavy chain (Abcam [ab70328], 1:1,000), rabbit anti-b2M (Life Technologies, 603 Carlsbad, CA [4H5L5], 1:1,000), rabbit anti-Skp1 (Cell Signaling Technology, Danvers, MA 604 [2156S], 1:750), or rabbit anti-Nrf1 (Cell Signaling Technology [D5B10], 1:1,000). .. Bound 605 primary antibodies were detected using horseradish peroxidase (HRP)-conjugated horse anti-606 mouse, anti-rabbit, and anti-rat IgG (Cell Signaling Technology, [7076S, 7074S, 7077S], 607 1:10,000).

Article Title: Orientia tsutsugamushi Ank5 promotes NLRC5 cytoplasmic retention and degradation to inhibit MHC class I expression.
Article Snippet: .. Blots were blocked in either 5% (vol/vol) non-fat dry milk or 5% (vol/vol) BSA in tris-buffered saline plus 0.05% Tween 20 (TBS-T) after which they were screened with rat anti-NLRC5 (MilliporeSigma [MABF260], 1:1000), rabbit antiTSA5665 (1:1000), rabbit or mouse anti-Flag (Sigma-Aldrich [F7425 or F1804], 1:1000), rabbit anti-GFP (ThermoFisher Scientific, Rockford, IL [A-6455], 1:1000), mouse anti-GAPDH (Santa Cruz, Dallas, TX [sc365062], 1:750), rabbit or mouse anti-myc (Abcam, Cambridge, United Kingdom [ab9106 and ab32], 1:1000), rabbit anti-HA (Abcam [ab9110], 1:4000),mouse anti-HLA-ABC heavy chain (Abcam [ab70328], 1:1000), rabbit anti-β2M (Life Technologies, Carlsbad, CA [4H5L5], 1:1000), rabbit anti-Skp1 (Cell Signaling Technology, Danvers, MA [2156S], 1:750), rabbit anti-Nrf1 (Cell Signaling Technology [D5B10], 1:1000), rabbit anti-lamin A/C (Cell Signaling [2032S]; 1:1000), or mouse antiHsp90 (Santa Cruz [13119]; 1:250). .. Bound primary antibodies were detected using horseradish peroxidase (HRP)-conjugated horse antimouse IgG, anti-rabbit IgG, and anti-rat IgG (Cell Signaling Technology, [7076S, 7074S, 7077S], 1:10,000).

Incubation:

Article Title: Empagliflozin Alleviates Hepatic Steatosis and Oxidative Stress via the NRF1 Pathway in High-Fat Diet-Induced Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease.
Article Snippet: .. The membranes were blocked with 5% (w/v) bovine serum albumin for 30 min and incubated overnight at 4 ◦C with the following primary antibodies: rabbit anti-NRF1 (#46743), rabbit anti-SIRT7 (#5360), and rabbit anti-αtubulin (#2144), each obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). ..

Article Title: Empagliflozin Alleviates Hepatic Steatosis and Oxidative Stress via the NRF1 Pathway in High-Fat Diet-Induced Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease
Article Snippet: .. The membranes were blocked with 5% ( w/v ) bovine serum albumin for 30 min and incubated overnight at 4 °C with the following primary antibodies: rabbit anti-NRF1 (#46743), rabbit anti-SIRT7 (#5360), and rabbit anti-α-tubulin (#2144), each obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). ..

Immunofluorescence:

Article Title: Chemogenetic profiling of ubiquitin-like modifier pathways identifies NFATC2IP as a mediator of SUMO-dependent genome integrity
Article Snippet: The following TaqMan probes were used in the experiment (detailed sequence information in Table S4): PSMA7 (Hs00895424_m1), PSMB7 (Hs00160607_m1), PSMC4 (Hs00197826_m1), and GAPDH (Hs99999905_m1). .. Primary antibodies used in this study were the following: rabbit anti-NRF1 (Cell Signaling Technologies, D5B10, cat # 8052, 1:1000), rabbit anti-GAPDH (Sigma, cat # G9545, 1:10000), mouse anti-NFATC2IP (Santa Cruz Biotechnology, B-1, cat # sc-377461, 1:100), rabbit anti-GFP (Abcam, cat # ab290, 1:1000), mouse anti-ɑ-tubulin (Cell Signaling Technologies, DM1A, cat # 3873, 1:2000), anti-centromere protein antibody (ACA) (Antibodies Incorporated, cat # 15-235, 1:1000), rat anti-FLAG (BioLegend, L5, cat # 637301, 1:500 for immunofluorescence), mouse anti-FLAG M2 (Sigma, cat # F1804, 1:500 for immunoblotting), rabbit anti-NSMCE2 (Novus Biologicals, cat # NBP1-76263, 1:1000), rabbit anti-SMC5 (Novus Biologicals, cat # NB100-469, 1:500), rabbit anti-RanGAP1 (Abcam, EPR3295, cat # ab92360, 1:2000), rabbit anti-SUMO1 (Abcam, Y299, cat # ab32058, 1:1000), rabbit anti-SUMO2/3 (Abcam, cat # ab3742, 1:1000), rabbit anti-Lamin B1 (Abcam, cat # ab16048, 1:1000), and rabbit anti-Histone H3 (Abcam, cat # ab1791, 1:4000). .. For immunoblotting, the following secondary antibodies were used: horseradish peroxidase (HRP)-conjugated AffiniPure goat anti-mouse IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., cat # 115-035-003, 1:5000), HRP-conjugated AffiniPure goat anti-rabbit IgG (H+L) (Jackson ImmunoResearch Laboratories Inc., cat # 111-035-144, 1:5000), IRDye 680RD donkey anti-mouse IgG (LI-COR Biosciences, cat # 926-68072, 1:5000), IRDye 680RD goat anti-mouse IgG (LI-COR Biosciences, cat # 926-68070, 1:5000), and IRDye 800CW donkey anti-rabbit IgG (LI-COR Biosciences, cat # 926-32213, 1:5000).

Western Blot:

Article Title: Orientia tsutsugamushi Ank5 directs ubiquitination and proteasomal degradation of NLRC5 to inhibit major histocompatibility complex class I expression
Article Snippet: .. Western blotting was performed as previously described.68 Briefly, normalized 593 amounts of eluates were resolved by SDS-PAGE in 4 to 15% TGX polyacrylamide gels (Bio-Rad) 594 at 110 V for 15 min followed by 200 V for 25 min. Proteins were transferred onto nitrocellulose 595 membrane in Towbin buffer at 100 V for 30 min. Blots were blocked in either 5% (vol/vol) non-596 fat dry milk or 5% (vol/vol) BSA in tris-buffered saline plus 0.05% Tween 20 (TBS-T) after which 597 they were screened with rat anti-NLRC5 (MilliporeSigma [MABF260], 1:1,000), rabbit anti-598 TSA5665 (1:1,000), rabbit or mouse anti-Flag (Sigma-Aldrich [F7425 or F1804], 1:1,000), rabbit 599 anti-GFP (ThermoFisher Scientific, Rockford, IL [A-6455], 1:1,000), mouse anti-GAPDH (Santa 600 Cruz, Dallas, TX [sc-365062], 1:750), rabbit or mouse anti-myc (Abcam, Cambridge, United 601 Kingdom [ab9106 and ab32], 1:1,000), rabbit anti-HA (Abcam [ab9110], 1:4,000), mouse anti-602 HLA-ABC heavy chain (Abcam [ab70328], 1:1,000), rabbit anti-b2M (Life Technologies, 603 Carlsbad, CA [4H5L5], 1:1,000), rabbit anti-Skp1 (Cell Signaling Technology, Danvers, MA 604 [2156S], 1:750), or rabbit anti-Nrf1 (Cell Signaling Technology [D5B10], 1:1,000). .. Bound 605 primary antibodies were detected using horseradish peroxidase (HRP)-conjugated horse anti-606 mouse, anti-rabbit, and anti-rat IgG (Cell Signaling Technology, [7076S, 7074S, 7077S], 607 1:10,000).

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